Chemical biology news from across RSC Publishing.
Chip-based gene transfer
19 December 2007
Dutch researchers have developed a highly efficient method of transferring DNA into stem cells using a lab on a chip style approach.
An electric pulse is used to create pores in single cells held in a microfluidic device
To improve the DNA take-up, or transfection, rate, Albert van den Berg and Ana Valero at the University of Twente, in Enschede, The Netherlands, and their co-workers have developed a system for transferring DNA into stem cells held in a microfluidic device. In this process individual cells are immobilised in mechanical traps and electroporated to transfer DNA into the cells. The added DNA encodes a fluorescent protein which is expressed, demonstrating that the DNA is still biologically viable. The transfection rate was high, with over 75 per cent of cells producing the protein, which van den Berg attributes to the system's 'very well controlled environment.' Looking to the future, van den Berg said the team hoped to create 'a tool for biologists to study and further develop cell reprogramming.'
Huabing Yin, an expert in applying microfluidic techniques to study biological systems, from the University of Glasgow, UK, welcomed the research. 'It demonstrates an efficient way of achieving gene transfer in a single stem cell,' said Yin, 'and is an excellent example of microfluidic applications in life science.'
Link to journal article
Gene transfer and protein dynamics in stem cells using single cell electroporation in a microfluidic device
A. Valero, J. N. Post, J. W. van Nieuwkasteele, P. M. ter Braak, W. Kruijer and A. van den Berg, Lab Chip, 2008, 8, 62
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