avian & livestock assay data sheet
- Ultrasensitive qualitative detection of bovine parvovirus by
real time polymerase chain reaction
polyomavirus (BPyV) is
a double-stranded DNA virus of genus Polyomavirus, of which
SV40, a DNA transforming virus, is also a member. The virus is
non-enveloped, with a diameter of 40-50 nm. Members of the
Polyomavirus genus usually contain circular dsDNA about 5 kb in
size, associated with cellular histones in a chromatin-like
complex. The viral genome encodes for 5-9 proteins. Occasionally
the viral genome can integrate into the genome of the host cell,
which can cause tumor formation in susceptible host animals.
Bovine polyomavirus was
originally found in fetal rhesus monkey kidney cells and was
thought to be simian polyomavirus until scientists confirmed
that it originated from the fetal calf serum that was used to
culture these cells. Genomic sequence for bovine polyomavirus has subsequently been detected
with high frequency in bovine sera, regardless of geographic
region of origin (Shuurman et al., 1991; Wang et al., 2005).
Culture detection of
this virus by cell culture method is not very sensitive and
requires a long time to complete. Detection by PCR is highly
sensitive, specific and rapid.
Help confirm the disease causing agent
Identify bovine polyomavirus carriers
Help ensure that herds and populations are free of
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive from
Schuurman, R., van
Steenis, B., van Strien, A., van der Noordaa, J., and Sol, C.
(1991) Frequent detection of bovine polyomavirus in commercial
batches of calf serum by using the polymerase chain reaction. J.
Gen. Virol. 72:2739-2745.
Wang, J., Horner, G.W., and
O’Keefe, J.S. (2005) Detection and molecular characterisation of
bovine polyomavirus in bovine sera in New Zealand. New Zealand
Vet. J. 53:26-20.
Specimen requirements: 0.2 ml whole blood
in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
Turnaround time: 2 business days
Methodology: Qualitative real time PCR
Normal range: Nondetected
Bovine polyomavirus PCR test