Thesis - Open Access
Master of Science (MS)
Department / School
Many growth regulators for obtaining various effects with plants have proven to be quite useful in industry, for weed control, and in investigations into the biochemical and physiological studies of plants. Went (1928) established the oat coleoptile curvature as a bio-assay test for growth regulators. Modifications were made to this original test; however each require elaborate manipulations and procedures in order to obtain definite results. In reviewing this test and others, and seeing the importance of growth regulators, it was questioned whether or not another, quicker, and easier test could be designed, which could supplement or compare to the oat coleoptile test. This paper is devoted to the investigation of this problem with the use of hypocotyl hooks of dark-grown cucumber seedlings, variety Marketeer. Three approaches were made in investigating the problem: Solution method (stock solution of TIBA made up at 100 ppm in water), Agar method (Agar prepared with 100 ppm trans-cinnamic acid), and Lanolin paste method (plants treated with lanolin paste at height range 7-10 cm).
Library of Congress Subject Headings
Includes bibliographical references
Number of Pages
South Dakota State University
Drangeid, Paul E., "The Use of Cucumber Seedlings to Measure Growth Regulator Activity" (1961). Electronic Theses and Dissertations. 2750.